John Lewis is to close its first store since 2006.
The retailer said the 35,000 sq ft Knight & Lee outlet in Southsea in Hampshire was its smallest full-range department store and could not easily be modernised.
John Lewis has previously acknowledged the "challenges" facing the High Street in the current retail climate.
However, it insisted it was not planning any other closures at the moment.
The store, which will close in July, is one of only two John Lewis shops in the UK to retain its original name.